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Genomic 5' end and 3' end FLAG gene-tagging in S. Pombe and S. Cerevisae using KanMX6
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Genomic 5' end and 3' end FLAG gene-tagging in S. Pombe and S. Cerevisae using bleMX6
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Image Search Results
Journal: bioRxiv
Article Title: The repurposed drugs suramin and quinacrine inhibit cooperatively in vitro SARS-CoV-2 3CL pro
doi: 10.1101/2020.11.11.378018
Figure Lengend Snippet: (A) 3D representation of the 3CL pro -quinacrine complex (3CL pro in Ribbon and quinacrine in Sticks). 3CL pro monomers are colored in green and blue, and quinacrine in orange. Normalized activity and inhibition of the virus proteases and, Lineweaver-Burk plots to determine the inhibition mode are presented. [S] is the substrate concentration; v is the initial reaction rate. (B) Normalized activity and inhibition of SARS-CoV-2 3CL pro under quinacrine influence. (C) Lineweaver-Burk plot for quinacrine inhibition of SARS-CoV-2 3CL pro .
Article Snippet:
Techniques: Activity Assay, Inhibition, Concentration Assay
Journal: bioRxiv
Article Title: The repurposed drugs suramin and quinacrine inhibit cooperatively in vitro SARS-CoV-2 3CL pro
doi: 10.1101/2020.11.11.378018
Figure Lengend Snippet: (A) 3D representation of the 3CL pro -suramin complex (3CL pro in Ribbon and suramin in Sticks). 3CL pro monomers are colored in green and blue and suramin in orange. Normalized activity and inhibition of the virus proteases and, Lineweaver-Burk plots to determine the inhibition mode are presented. [S] is the substrate concentration; v is the initial reaction rate. (B) Normalized activity and inhibition of SARS-CoV-2 3CL pro under suramin influence. (C) Lineweaver-Burk plot for suramin inhibition of SARS-CoV-2 3CL pro .
Article Snippet:
Techniques: Activity Assay, Inhibition, Concentration Assay
Journal: bioRxiv
Article Title: The repurposed drugs suramin and quinacrine inhibit cooperatively in vitro SARS-CoV-2 3CL pro
doi: 10.1101/2020.11.11.378018
Figure Lengend Snippet: (A) Ribbon and sticks representation of the SARS-CoV-2 3CL pro -quinacrine complex after MD simulation. (B) Decomposition of the binding energy of SARS-CoV-2 3CL pro -quinacrine complex. Arrows label protomer A (turquoise) and protomer B (gold). (C) SARS-CoV-2 3CL pro amino acids involved in the interaction with quinacrine based on MD simulations. (D) Quinacrine interaction in the protease substrate-binding and active site. The substrate-binding subsites are highlighted: green S1’: gold S1; red S2 and blue S3.
Article Snippet:
Techniques: Binding Assay
Journal: bioRxiv
Article Title: The repurposed drugs suramin and quinacrine inhibit cooperatively in vitro SARS-CoV-2 3CL pro
doi: 10.1101/2020.11.11.378018
Figure Lengend Snippet: (A) Ribbon and sticks representation of the SARS-CoV-2 3CL pro -Suramin complex after MD simulation. (B) Decomposition of the binding energy of SARS-CoV-2 3CL pro -suramin complex. Arrows label protomer A (turquoise) and protomer B (gold). (C) SARS-CoV-2 3CL pro amino acids involved in the interaction with Suramin based on MD simulations.
Article Snippet:
Techniques: Binding Assay
Journal: bioRxiv
Article Title: The repurposed drugs suramin and quinacrine inhibit cooperatively in vitro SARS-CoV-2 3CL pro
doi: 10.1101/2020.11.11.378018
Figure Lengend Snippet: Protomer 1 is colored in turquois, protomer 2 in grey and active site pocket volume in violet. Red arrows highlight a conformational change in the SARS-CoV-2 3CL pro /suramin complex. (A) SARS-CoV-2 3CL pro dimer. (B) SARS-CoV-2 3CL pro /suramin complex. (C) Active site pocket volume of SARS-CoV-2 3CL pro , substrate-binding subsites are highlighted as green S1’; gold S1; red S2 and blue S3. (D) Active site pocket volume of protomer 2. (E) Active site pocket volume of protomer 1.
Article Snippet:
Techniques: Binding Assay
Journal: bioRxiv
Article Title: The repurposed drugs suramin and quinacrine inhibit cooperatively in vitro SARS-CoV-2 3CL pro
doi: 10.1101/2020.11.11.378018
Figure Lengend Snippet: (A) Ribbon representation of the SARS-CoV-2 3CL pro model (grey/cyan) (PDB entry: 6M2Q) in complex with suramin and quinacrine (sticks in orange). (B) Normalized activity and inhibition of SARS-CoV-2 3CL pro under quinacrine and suramin influence.
Article Snippet:
Techniques: Activity Assay, Inhibition